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Q12 Reagents

Q12 Reagent is a long-chain ion-pair concentrate used in reversed-phase HPLC to enhance retention and selectivity for suitable charged pharmaceutical compounds.

Q12 ion pair concentrate bottles for HPLC reagent applications

What is Q12 Reagent?

Q12 Reagent is a long-chain ion-pair concentrate used in reversed-phase HPLC to enhance retention and selectivity for suitable charged pharmaceutical compounds.

Q12 chemistry supports retention enhancement, selectivity improvement and reproducible ion-pair chromatography when mobile phase preparation, concentration and pH are controlled carefully.

Technology, mechanism and applications

Q12 chemistry supports retention enhancement, selectivity improvement and reproducible ion-pair chromatography when mobile phase preparation, concentration and pH are controlled carefully.

  • Method development and replacement screening support
  • Pharmaceutical QC, impurity profiling and research laboratories
  • Typical analytes include APIs, intermediates, ionic compounds, racemates and polar analytes depending on family
  • Storage and care: use compatible solvents, filter mobile phases and follow validated laboratory procedures.

Technical support

For product selection, dimensions and method transfer support, contact Akira Analytical Solutions Pvt. Ltd..

SpecificationsApplicationsUSP ListingContact

Available catalog specifications

Product nameChemistryParticle sizeDimensionCatalog number
ION PAIR CONCENTRATE Q12 (100 mL)ION PAIR CONCENTRATE Q12N/AN/A1-404031-200

Frequently asked questions

What is Q12 used for?

Q12 is used to improve reversed-phase retention and selectivity for suitable charged analytes.

How should Q12 concentration be optimized?

Begin with method-development screening and adjust concentration only after pH and organic solvent are controlled.

How does Q12 differ from shorter ion-pair reagents?

Long-chain reagents generally provide stronger hydrophobic contribution and can increase retention.

What troubleshooting is important for Q12?

Equilibrate the column thoroughly, maintain consistent pH and avoid reagent carryover between incompatible methods.

Who supplies Q12 reagent in India?

Akira Analytical Solutions Pvt. Ltd. supplies Q12 products in India.

Technical Reference

Dodecyltriethylammonium Phosphate (Q12) is a long-chain quaternary ammonium ion-pair reagent used in reversed-phase HPLC to improve retention and selectivity for acidic and ionic analytes. The hydrophobic alkyl chain adsorbs onto the stationary phase while the positively charged ammonium group forms ion pairs with negatively charged analytes, enhancing chromatographic retention and peak shape.

Pentanesulfonic Acid Sodium Salt (PSA), Hexanesulfonic Acid Sodium Salt (HSA), Heptanesulfonic Acid Sodium Salt, and Octanesulfonic Acid Sodium Salt (OSA) are anionic ion-pair reagents commonly used for basic pharmaceuticals, alkaloids, amines and catecholamines. Increasing alkyl chain length generally increases hydrophobic interaction and retention.

Tetrabutylammonium Hydrogen Sulfate (TBAHS) is widely used for acidic compounds, nucleotides and organic acids, providing reproducible retention on C18 phases. Method development typically begins at 2–10 mM reagent concentration with pH selected according to analyte pKa.

Best Practices